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. 2017 May 23;7:2283. doi: 10.1038/s41598-017-02276-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of mutations on infectivity. Transduction efficiency of 293 T cells using WT and mutant vectors at a MOI of 1 was determined by measure of EGFP expression by flow cytometry (a) and by qPCR to quantify total and integrated viral DNA (b). The results derived from the mean of at least three independent experiments. Student’s t-test *p < 0.05.