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. 2017 May 23;7:2298. doi: 10.1038/s41598-017-02683-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Glucose induces FAM3B expression in VSMCs. Cultured rat VSMCs were stimulated with 25 mM glucose for the indicated time-points. (A) RT-qPCR and (B) Western blotting analyses of FAM3B expression levels. ^* P < 0.05 and ^** P < 0.01 vs. 0 h. Cultured VSMCs were stimulated with indicated concentrations of glucose for 36 h. (C) RT-qPCR and (D) Western blotting analyses of FAM3B expression levels. ^* P < 0.05 and ^** P < 0.01 vs. 5.5 mM glucose. (E) Quantification of the half-life of FAM3B protein by a CHX chase experiment. (F) Ubiquitination of FAM3B protein.