Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 May 23;7:2298. doi: 10.1038/s41598-017-02683-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Overexpression of FAM3B accelerates VSMC proliferation and migration. VSMCs were infected with recombinant adenoviruses expressing either EGFP (as negative control) or FAM3B for 48 h in DMEM medium containing 5.5 mM glucose. Cell proliferation was evaluated using the EdU incorporation (A,B) or direct cell counting assay (C). ^* P < 0.05 and ^** P < 0.01 vs. Ad-EGFP group. (D) Assessment of the cell cycle progression by flow cytometry. VSMCs were similarly infected with adenoviruses in DMEM medium containing 5.5 mM or 25 mM glucose, respectively. ^* P < 0.05 and ^** P < 0.01 vs. Ad-EGFP/5.5 mM glucose; ^## P < 0.01 vs. Ad-EGFP/25 mM glucose. (E) Protein expression levels of key regulators involved in cell cycle progression. (F) Determination of VSMC migration by transwell chamber (top) and wound-healing (bottom) assays. (G,H) Quantification of the data from (F). ^** P < 0.01 vs. Ad-EGFP group. (I) Protein expression levels of ICAM-1, VCAM-1, and MMPs. (J,K) Total activity of MMP-9 and MMP-2. ^** P < 0.01 vs. Ad-EGFP group.