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. 2017 May 24;8:600. doi: 10.3389/fimmu.2017.00600

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Copyright © 2017 Hobbs and Nolz.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Binding properties of lectins used to interrogate core 1 O-glycan status. Jacalin can bind the unmodified core 1 base regardless of whether it is sialylated. Peanut agglutinin (PNA) will only bind core 1 O-glycans when the α2,3-sialic acid is not present. Maackia amurensis lectin II (MAL II) reacts to the α2-3 sialic acid linked to the β1,3-galactose of core 1 O-glycans. Together, this panel of lectins can determine if core 1 contains the sialic acid cap (Jacalin⁺, MAL II⁺) and whether it is possible that core 2 is present (core 2 requires unmodified core 1 as a substrate and therefore can only be present on PNA⁺ and MAL II^–cells).