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. 2017 May 24;8:595. doi: 10.3389/fimmu.2017.00595

Figure 2.

Figure 2

Antigen (Ag)-specific humoral responses in human serum samples following homologous prime-boost vaccination regimens. Participants received 3× priming immunizations (IMs) of CN54gp140 adjuvanted with GLA-AF on days 0, 28, and 56, followed by a booster injection at day 168 (Group A) or day 336 (Group B). Indicated by dotted line and numbers 1°–4°. (A,B) CN54gp140-specific IgG, IgA, IgM and (C,D) IgG-subclass concentrations were measured in human serum/plasma and expressed as mean ± SEM. (E) The sum of the four IgG subclasses was calculated and used to determine the percentage of CN54gp140-specific IgG-subclass antibodies 14 days after the priming phase (D70) and 14 days after the respective booster injections (D182/D350) for both Groups A and B. The proportion of Ag-specific IgG1 was decreased, while Ag-specific IgG4 levels were significantly increased after the respective booster IMs. Non-parametric Mann–Whitney test (*p < 0.05, **p < 0.01).