TABLE 2.

Analysis of variance for ARISA profile richness^a

Sensitivity cutoff and variable	dfb	MSc	F statistic	P
50 FU
Mo	2	115.0	0.78	0.460
Region	1	8,020.4	54.69	<<0.001***
LP	1	1,380.3	9.41	0.003*
Mo-region	2	325.0	1.11	0.335
Mo-LP	2	448.1	1.53	0.223
Region-LP	1	556.3	3.86	0.053
Mo-region-LP	1	52.2	0.36	0.553
Residual	79	146.6
100 FU
Mo	2	121.4	0.86	0.458
Region	1	9,945.2	70.28	<<0.001***
LP	1	1,253.6	8.86	0.004*
Mo-region	2	140.1	0.99	0.376
Mo-LP	2	235.1	1.66	0.196
Region-LP	1	680.3	4.81	0.031
Mo-region-LP	1	5.7	0.04	0.842
Residual	79	141.5
150 FU
Mo	2	139.6	1.10	0.339
Region	1	9,608.6	75.55	<<><0.001***
LP	1	1,391.5	10.94	0.001**
Mo-region	2	280.5	1.10	0.337
Mo-LP	2	484.7	1.91	0.155
Region-LP	1	524.3	4.12	0.046*
Mo-region-LP	1	9.2	0.07	0.789
Residual	79	10,046.8
200 FU
Mo	2	112.2	0.95	0.391
Region	1	9,252.6	78.36	<<0.001***
LP	1	1,244.0	10.53	0.002**
Mo-region	2	145.9	1.24	0.296
Mo-LP	2	233.4	1.98	0.145
Region-LP	1	358.4	3.04	0.085
Mo-region-LP	1	9.8	0.08	0.774
Residual	79	118.1

^aTests were carried out for each level of ARISA sensitivity, using only peaks exceeding the reported fluorescence cutoff. All significance levels have been Bonferroni corrected (α′ = α/4). Mo refers to the month of sample collection (May, July, or October); region refers to north versus south; LP refers to seepage versus drainage. Significance levels: *, α = 0.05; **, α = 0.01; ***, α = 0.001.

^bdf, degrees of freedom.

^cMS, mean square.