Table 1.
Enzyme | E.C. | Abbreviation | Activity (μmol min-1 g-1) |
---|---|---|---|
Triose phosphate isomerase | 5.3.1.1 | TPI | 5680 – 7190 |
UDP-glucose pyrophosphorylase | 2.7.7.9 | UDPase | 275 – 489 |
Glucose 6-phosphate isomerase | 5.3.1.9 | G6PI | 194 – 328 |
Phosphoglucomutase | 5.4.2.2 | PGM | 92 – 113 |
Pyruvate kinase | 2.7.1.40 | PK | 62 – 80 |
Enolase | 4.2.1.11 | ENO | 25 – 34 |
Phosphoglycerate kinase | 2.7.2.3 | PGK | 12 – 22 |
Aldolase | 4.1.2.13 | ALD | 13 – 15 |
Phosphoglycerate mutase | 2.7.5.3 | PGlyM | 10 – 16 |
ATP-dependent phosphofructokinase | 2.1.7.11 | PFK | 4.3 – 5.6 |
Glyceraldehyde 3-phosphate dehydrogenase | 1.2.1.12 | GAPDH | 3.4 – 4.3 |
Phosphoenolpyruvate phosphatase | 3.1.3.60 | PEPase | 2.5 – 3.0 |
Hexokinase | 2.7.1.1 | HK | 1.6 – 2.3 |
Fructokinase | 2.7.1.4 | FK | 1.0 – 1.3 |
Pyrophosphate-dependent phosphofructokinase | 2.7.1.90 | PFP | 0.65 – 1.0 |
Activities are presented as the range of activities that occurred during 60 days storage (lowest observed activity to highest observed activity), using the mean activities from samples at 0, 1, 2, 3, 4, 7, 10, 30, and 60 days after harvest. Enzymes are listed in order of decreasing mean activity, with activity expressed as the quantity of substrate consumed min-1 g-1 protein. Roots were stored 10 days at 10°C and 90 ± 5% relative humidity (RH), then stored at 4°C, 90 ± 5% RH for 50 days.