TABLE 4.
Toxicity and cross-resistance of Cry11A, Cyt1A, or Cry11A plus Cyt1A against C. quinquefasciatus after selection for approximately 48 generations with one or two toxins of B. thuringiensis subsp. israelensis
| Mosquito straina | Test toxin(s) | LC50 (μg/ml), FLb | LC95 (μg/ml), FL | Resistance ratio at:
|
|||
|---|---|---|---|---|---|---|---|
| LC50 | LC95 | SF50 | SF95 | ||||
| SynParent | Cyt1A | 25.4, 14.1-45.8 | 280, 93.8-842 | 1.0 | 1.0 | ||
| Cyt1A | Cyt1A | 53.9, 34.0-85.6 | 1,340, 487-3,874 | 2.1 | 4.8 | ||
| Cry11A | Cyt1A | 31.3, 16.3-60.1 | 356, 97.2-1,323 | 1.2 | 1.3 | ||
| Cry11A + Cyt1Aa | Cyt1A | 48.8, 20.9-113 | 692, 132-3,671 | 1.9 | 2.5 | ||
| SynParent | Cry11A | 0.946, 0.419-2.13 | 24.9, 5.57-112 | 1.0 | 1.0 | ||
| Cyt1A | Cry11A | 0.904, 0.424-1.92 | 17.6, 4.44-70.5 | 0.9 | 0.7 | ||
| Cry11A | Cry11A | 18.1, 11.0-29.8 | 1,592, 469-5,723 | 19.1 | 63.9 | ||
| Cry11A + Cyt1A | Cry11A | 4.48, 3.59-5.54 | 231, 155-374 | 4.7 | 9.3 | ||
| SynParent | Cry11A + Cyt1A | 0.209, 0.158-0.278 | 0.832, 0.505-1.42 | 1.0 | 1.0 | 6.0 | 38.8 |
| Cyt1A | Cry11A + Cyt1A | 0.187, 0.107-0.321 | 0.671, 0.252-1.95 | 0.9 | 0.8 | 6.4 | 34.8 |
| Cry11A | Cry11A + Cyt1A | 0.476, 0.422-0.536 | 1.87, 1.53-2.40 | 2.3 | 2.2 | 42.5 | 455 |
| Cry11A + Cyt1A | Cry11A + Cyt1A | 1.07, 0.927-1.24 | 7.30, 5.74-9.84 | 5.1 | 8.8 | 5.4 | 20 |
a
SynParent, nonselected; Cyt1A, Cyt1A selected; Cry11A, Cry11A selected; Cry11A + Cyt1A, Cry11A plus Cyt1A selected.
b
FL, fiducial limits.