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. 2017 May 24;7:95. doi: 10.3389/fonc.2017.00095

Figure 2.

Figure 2

Combination treatment with the mutant BRAF inhibitor vemurafenib and histone deacetylase inhibitors (HDACi). BRAF wild-type MEWO and BRAF-mutant A375 and A2058 cells were treated with the mutant BRAF inhibitor vemurafenib (0.5 µM), HDACi SAHA (1 µM), and valproate (2 mM) alone or in combination for 48 h. After Western blotting expression of PMCA4b, PMCA1, SERCA2, SERCA3, pERK, and ERK proteins were analyzed. As a positive control of PMCA1 and PMCA4b, total cell lysate of COS-7 cells was used, which endogenously express both proteins (9). (A) The expression of PMCA proteins was detected with isoform-specific and pan-PMCA antibodies. (B) After densitometric analysis, changes in PMCA4b (B), ERK (C) and SERCA2 (D) protein levels were normalized to the expression levels of β-tubulin and expressed as fold increase over the untreated controls. Bars represent mean ± SE from three independent experiments. Significance compared to control is denoted by asterisks (*p < 0.05), using two-tailed paired t-test.