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. 2017 May 24;8:850. doi: 10.3389/fpls.2017.00850

FIGURE 4.

FIGURE 4

Spatio-temporal profile of VvDELLA proteins in V. vinifera cv. Black finger (BF) and cv. Spring blush (SB). Blots of total protein extracted from internodes (A), rachises (B), leaves (C), tendrils (D), pistils and berries (E) at different developmental stages. Sampling of the tissues was carried out in 2010. Blots were incubated with affinity-purified, gene-specific, anti-VvDELLA polyclonal antibodies. Recombinant full-length proteins (R.P.) (3.75 ng each of VvDELLA1 and VvDELLA2 and 37.5 ng of VvDELLA3) were used as sizing controls. Coomassie Brilliant Blue-stained (CBB) proteins were used as loading control. In all lanes except R.P., solid black arrows show band of interest, and asterisked-bands (∗) indicate non-specific proteins detected by the anti-VvDELLA antibodies. Differences in sizes of R.P. and endogenous VvDELLA proteins result from V5 and 6xHis tags on the R.P. 0 d, berries sampled at 2–3 mm diameter (E-L 27); 10 d, berries sampled 10 days after E-L 27; 30 d, berries sampled 30 days after E-L 27. Similar results were obtained when samples collected in 2011 growing season were analyzed.