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. 2016 Aug 23;21(5):1777–1788. doi: 10.1007/s00784-016-1946-5

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© The Author(s) 2016

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 1 — Representative electrophoregrams for the analysis of CXXC4, DACT2, FBXW11, HDPR1, HHIP, PTCH1, SUFU, ZIC1, and ZIC4 methylation by methylation-specific PCR using starters specific for the methylated sequence (M) and starters specific for the unmethylated sequence (U) in head and neck squamous cell carcinoma cell lines and control DNA samples: HOK DNA isolated from healthy oral keratinocytes, MET completely methylated human DNA, WBC DNA derived from white blood cells of healthy blood donors