. 2016 Aug 23;21(5):1777–1788. doi: 10.1007/s00784-016-1946-5

Table 4.

The sequence of starters used in real-time PCR analysis

Gene	Starter sequence		Amplicon size	Annealing T
CXXC4	F	5′ CACCTCCTCCTCCTTCCAC	105 bp	56 °C
CXXC4	R	5′ GCATTATCCTCCAACTGTTACAAC	105 bp	56 °C
DACT2	F	5′ CAGGCTTTTACGAGATGAG	89 bp	56 °C
DACT2	R	5′ CAGCGAGGGAGAGATGTG	89 bp	56 °C
HHIP	F	5′ TCCTATACCACCAACCAAGAAC	165 bp	59 °C
HHIP	R	5′ TTGTCCTCCCAGATGCTTTC	165 bp	59 °C
PBGD	F	5′ TCAGATAGCATACAAGAGACC	111 bp	56 °C
PBGD	R	5′ TGGAATGTTACGAGCAGTG	111 bp	56 °C

F forward primer, R reverse primer