Table 4.

Methanogenic species relative abundance proportions, based on 16S rDNA reads taxonomic classification, in the rumen and cecum of bulls receiving a control (CTL) or linseed plus nitrate (LINNIT) supplemented diet.

	CTL		LINNIT		SEM	Effect
	Rumen	Cecum	Rumen	Cecum		Treatment	DCa	Treatment × DC
Methanobrevibacter gottschalkii	0.360	0.504	0.439	0.596	0.046	0.093	0.005	0.888
Methanobrevibacter ruminantium	0.198	0.198	0.154	0.085	0.030	0.013	0.260	0.254
unclassified Group9	0.133	0.123	0.113	0.176	0.032	0.651	0.484	0.336
Group10 sp	0.123	–	0.138	–	0.014	0.713	0.000	0.713
Methanosphaera sp ISO3-F5	0.044	0.024	0.053	0.013	0.009	0.924	0.005	0.340
Group12 sp	0.036	–	0.028	0.000	0.004	0.505	0.000	0.499
unclassified Methanosphaera	0.033	0.006	0.014	0.009	0.009	0.457	0.136	0.306
Group9 sp	0.021	0.000	0.007	0.000	0.003	0.228	0.015	0.222
unclassified Methanobrevibacter	0.018	0.013	0.016	0.023	0.004	0.413	0.835	0.230
Methanobrevibacter boviskoreani	0.013	0.014	0.012	0.018	0.007	0.858	0.604	0.739
Methanosphaera stadtmanae	0.008	0.001	0.020	0.003	0.003	0.053	0.001	0.126
Group8 sp	0.005	0.116	0.001	0.067	0.016	0.252	0.001	0.329
Methanobacterium alkaliphilum	0.003	–	0.001	0.009	0.002	0.155	0.363	0.033
Group11 sp	0.003	–	0.001	–	0.001	0.225	0.078	0.225
unclassified_Methanobacteriaceae	0.000	0.000	0.000	0.000	0.000	0.457	0.136	0.306
Methanobrevibacter oralis	0.000	0.000	0.000	0.000	0.000	0.211	0.289	0.307
unclassified Methanomicrobiaceae	0.000	–	–	–	0.000	0.326	0.326	0.326
unclassified	0.000	–	0.000	–	0.000	0.937	0.169	0.937
Methanobacterium formicicum	–	–	0.000	0.000	0.000	0.277	0.388	0.388
Methanimicrococcus blatticola	–	–	0.000	–	0.000	0.326	0.326	0.326

^adigestive compartment.

Values are means of eight individual measures; data were analyzed using two-way ANOVA in R.