Fig. 1.

Schematic representation of strain construction. The auxotrophic strain Po1d (Leu−Ura−) was derived from WT strain W29. The construction of strains JMY195 (auxotrophic WT), JMY1233 (pox1-6Δ), JMY1877 (dga1Δ dga2Δ lro1Δ are1Δ [Q4]), and JMY2159 (pox1-6Δ dga1Δ lro1Δ dga2Δ fad2Δ) are described elsewhere (Barth and Gaillardin 1996; Beopoulos et al. 2008, 2012, 2014). The gray boxes contain intermediary strains, and the blue boxes contain study strains. Marker excision was performed with the replicative plasmid pUB4-CreI (pRRQ2). For FAD2 and oPAI overexpression, coding genes were expressed under the pTEF or pPOX promoter in the vector JMP62 containing either the URA3ex or LEU2ex excisable auxotrophic markers