Agarose gel electrophoresis of ClaI-digested plasmid DNAs representing nine different PFGE types (A) and vanY-vanZ hybridization analysis (B). Lanes 1 and 15, 1-kb DNA ladder (Sigma-Aldrich, Oslo, Norway); lane 2, long-PCR product of Tn1546 PCR (positive control); lane 3, 399/F98/H1 (PFGE type 1); lane 5, 399/F99/H8 (PFGE type 7); lane 6, 399/F98/A4 (PFGE type 5); lane 7, 399/S99/A5 (PFGE type 9A); lane 9, 64/F98/H1 (PFGE type 13); lane 10, 64/S99/H3 (PFGE type 15); lane 11, 64/F99/H4 (PFGE type 16); lane 13, 64/F98/A3 (PFGE type 20); lane 14, 64/F99/A5 (PFGE type 21); lanes 4, 8, and 12, failed plasmid isolations.