TABLE 2.
PCR primers used for this study
| PCR assay | Primer | Sequence(5′-3′) | Product size (bp) | Reference |
|---|---|---|---|---|
| Tn1546a | ORF1 F9 | AAC CTA AGG GCG ACA TAT GGT G | 10,414 | 21 |
| vanZ R11 | GGT ACG GTA AAC GAG CAA TAA TAC G | |||
| vanAb | vanAF | GTT GCA ATA CTG TTT GGG GG | 1,013 | 44 |
| vanAR | CCC CTT TAA CGC TAA TAC GAT CAA | |||
| vanX | vanX1 | ACT TGG GAT AAT TTC ACC GG | 424 | 44 |
| vanX2 | TGC GAT TTT GCG CTT CAT TG | |||
| vanY-vanZb | vanYF1 | ATG GAT ACG GGT TGC TTG ATA T | 1,375 | 44 |
| vanZR1 | TTT CCC CTC ACT TCA VCAC CTA C | |||
| Tn1546-Pb | vanZF | AGT GCT GAG GAA TTG GTC TCT | 422 | This study |
| P2 R | CCG AGA AAG CTG GTT AAG TCT A | |||
| ORF18-19 (pRE25 PSK)b | ζ-F | GTG GTT TAG GTG GCT GCA AG | 1,044 | This study |
| ɛ-R | TTA ACG AAT TAT CGG CAA GC | |||
| tcrB | 415 | TGA CAA TAA GGC AAC GAT TTC | 871 | 23 |
| 416 | CCA GGC ATG ATG TCC TTG |
a
Amplicon used as a positive control for Tn1546 hybridizations.
b
Amplicon used as a probe.