TABLE 3.
Distribution, relative numbers, and characteristics of GREF PFGE types in this study
| Farm | Origin | PFGE type(s) | No. of isolates
|
Transferable glycopeptide resistance | Common Tn1546 junction fragment | pRE25 PSK PCR | Cohybridization Tn1546-pRE25 PSK | ||
|---|---|---|---|---|---|---|---|---|---|
| Fall 1998 | Spring 1999 | Fall 1999 | |||||||
| 399 | Farmer | 1 | 7 | + | + | + | −a | ||
| 2 | 1 | + | + | − | |||||
| 3 | 2 | + | − | + | + | ||||
| 4 | 1 | + | + | + | + | ||||
| 5A | 11 | 15 | + | + | + | + | |||
| 6 | 3 | − | + | − | |||||
| 7 | 1 | + | + | + | + | ||||
| 8 | 2 | − | + | − | |||||
| Poultry | 9, 9A | 4 | 1 | + | + | + | − | ||
| 10, 10A | 4 | 7 | 4 | − | + | + | +b | ||
| 2 | 1 | + | + | − | |||||
| 5, 5A | 1 | 2 | + | + | + | +b | |||
| 11 | 1 | 3 | − | + | + | + | |||
| 12 | 1 | + | + | − | |||||
| 64 | Farmer | 13 | 5 | − | + | − | |||
| 14 | 8 | − | + | − | |||||
| 15 | 12 | 2 | − | + | − | ||||
| 16 | 8 | − | + | + | + | ||||
| 17 | 2 | − | + | − | |||||
| 18 | 1 | + | + | − | |||||
| Poultry | 10B | 8 | − | + | − | ||||
| 19 | 1 | + | + | − | |||||
| 20 | 1 | − | + | − | |||||
| 15 | 6 | 6 | − | + | − | ||||
| 21 | 3 | + | + | − | |||||
| 22 | 1 | − | − | − | |||||
a
vanY-vanZ and pRE25 PSK probes revealed positive signals for different PstI fragments.
b
The presence of pRE25 PSK was not a constant feature in PFGE types 5 and 10. The presence of pRE25 PSK and its physical linkage to Tn1546 were demonstrated when they were recovered as PFGE types 5A and 10A in spring and fall 1999.