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. 2017 May 17;8:15286. doi: 10.1038/ncomms15286

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Copyright © 2017, The Author(s)

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(a) Tetrad dissection of cut7Δpkl1Δ cells crossed to klp9Δ cells. (b) Time-lapse images of wild-type (wt) and cut7Δpkl1Δ cells expressing mCherry-Atb2 and Klp9-GFP from mitosis onset to spindle breakdown. Each frame corresponds to 3 min interval. (c) Time-lapse images of cut7Δpkl1Δ and cut7Δpkl1Δklp9^Off cells expressing mCherry-Atb2 and Sid4-GFP from mitosis onset to spindle breakdown, after 8 h of repression/shut-off. Each frame corresponds to 3 min interval. (d) Comparative plot of spindle length dynamics cut7Δpkl1Δ (red curves; n=16) and cut7Δpkl1Δklp9^Off cells (blue curves; n=18) after 8 h of repression/shut-off. Dotted vertical line corresponds to phase I–II transition; dashed vertical line corresponds to phase II–III transition. (e) Bar plot showing metaphase spindle length at anaphase transition, reported by Cdc13-GFP degradation, of wild-type (green; n=43), cut7Δpkl1Δ (red; n=36), cut7Δpkl1Δklp9^Off cells after 8 h repression/shut-off (blue; n=26), and cut7Δpkl1Δklp9^OE cells after 20 h of overexpression induction (orange; n=37). ns, not statistically significant. (f) Bar plot showing anaphase transition time of wild-type (green; n=43), cut7Δpkl1Δ (red; n=36), cut7Δpkl1Δklp9^Off cells after 8 h incubation in thiamine-containing medium (blue; n=26) and cut7Δpkl1Δklp9^OE cells after 20 h of overexpression induction (orange; n=37). NS, not statistically significant. (g) Bar plot showing anaphase spindle elongation velocity of wild-type (green; n=29), cut7Δpkl1Δ cells (red; n=22) and cut7Δpkl1Δklp9^OE cells after 20 h of overexpression induction (orange; n=29). *P<0.001. (h) Comparative plot of spindle length dynamics of cut7Δpkl1Δ (red curves; n=20) and cut7Δpkl1Δklp9^OE cells (orange curves; n=20) after 20 h of overexpression induction. Time 0 represents the start of anaphase elongation. (i) Comparative plots of spindle length dynamics of cut7Δpkl1Δ (red curves; n=22) and cut7Δpkl1Δklp2Δklp6Δklp5Δ cells (purple curves; n=22). Dotted vertical line corresponds to phase I–II transition; dashed vertical line corresponds to phase II–III transition. Data show mean±s.d. and Student's t-test P value. Scale bars, 2 μm.