Figure 2.

Survival curve and behavioral profile evaluation of mice infected with Crytococcus gattii and subjected to different treatments. (A) Six mice per group were inoculated with 10⁴ cells of L27/01 strain by intratracheal line inoculation and treated with fluconazole at 20 mg.kg⁻¹ (FCZ), pCramoll at 1 ug (pC1), or the combination (pC1 + FCZ). Animals treated by pC1 + FCZ had an average increase of over 34.8% in survival compared to those treated with FCZ alone ^*p < 0.05. (B–G) Five animals per group were submitted to the SHIRPA Protocol. There was no behavioral difference between uninfected mice (NI) and those treated with pC1 alone, which did also not influence the survival of mice. Untreated mice (NT); ^*p < 0.05; ^**p < 0.001 (difference when compared to NI); ⁺p < 0.5; ⁺⁺p < 0.001 (difference when compared to NT); ^#p < 0.05 (difference between FCZ and combination). Data represent the means ± SE from three independent experiments.