Figure 1.

Caspase-2 deficiency enhances aneuploidy and delays apoptosis in primary splenocytes. (a) Protocol used for time-lapse microscopy, cell viability and aneuploidy analysis in primary splenocytes. (b) Quantitation of metaphases showing frequencies of diploid and aneuploid karyotypes in untreated, PLK1 inhibitor (PLK1-I) treated and PLK1-I combined with z-VAD-fmk (pan caspase inhibitor) treated WT (n=4) and Casp2^−/− (n=4) splenocytes. A total of 50 metaphase spreads were counted per mouse. P values are indicated with **P<0.01, ***P<0.001, χ² test. (c) Quantitation of viable cells in WT and Casp2^−/− splenocytes treated with PLK1-I for the indicated time points, determined by trypan blue assay. Data represented as mean±s.e.m. from four independent experiments. P values are indicated for PLK1-I treated samples with **P<0.01.