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. 2016 Nov 28;36(20):2835–2845. doi: 10.1038/onc.2016.434

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Copyright © 2017 The Author(s)

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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CDK3 phosphorylates NFAT3 at Ser259. (a) GST-tagged truncated NFAT3 was used for an in vitro kinase assay with active CDK3. The phosphorylation was visualized by autoradiography (³²P-NFAT3). Coomassie blue staining was used to verify equal loading of GST-tagged truncated NFAT3 proteins (labeled with *). (b) Sequence of NFAT3 from amino acids 111–260. The predicted SP motifs are indicated with black boxes. (c) GST-tagged wildtype NFAT3 fragments or GST-tagged mutant NFAT3 fragments were used for an in vitro kinase assay with active CDK3 as indicated (upper panel). The phosphorylation was visualized by autoradiography (³²P-NFAT3) and Coomassie blue staining indicated the CDK3 protein and respective NFAT3 fragments (indicated as NFAT3 and CDK3).