Figure 4.

Induced expression of Zeb2 in inflamed colon downregulates the expression of its targets Cdh1 and miR-200b. The relative gene expression of Zeb2 (a) is significantly induced in inflamed colons as compared to both control and uninflamed colons of colitis-induced animals. In contrast, epithelial markers Cdh1 (b) and miR-200b (c) are both significantly downregulated in inflamed colons of colitis-induced animals. Data are presented as the mean ± SEM; the significance of differences between sets of data was determined by one-way analysis of variance (ANOVA) using SPSS Statistics; ^∗p < 0.05.