Table 1.
SNP ID | Primer sequence | Major/minor gene |
Product (bp) |
Annealing temperature (°C) |
Restriction enzyme |
Allele (bp) |
---|---|---|---|---|---|---|
rs4758680 | F:5′-GATCACCCGGACTCAAAACGTG-3' | C/A | 263 | 60 | MboII | A (263) |
R: 5′-TTGTGCAAACCACACCTCTTCG-3' | — | — | — | — | C (210 + 53) | |
| ||||||
rs7977932 | F:5′-GGTCAGTGTTGGGTTTGCAATG-3' | C/G | 121 | 60 | ScrFI | G (74 + 57) |
R:5′-TTGGTGATGGCACAGCCTCATA-3' | — | — | — | — | C (131) |