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. 2017 Mar 17;24(6):997–1006. doi: 10.1038/cdd.2017.31

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Copyright © 2017 The Author(s)

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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Intrahepatic Th1 cells were linked to the progress of liver fibrosis in BA. (a) Successive liver sections were used for Masson's trichrome staining to assess the stage of liver fibrosis. Data shown are representative of 32 early-stage BA patients with liver fibrosis grades I to IV. (b) Immumohistochemical staining was performed to detect T-bet⁺ cells, which specifically identifies Th1 cells.Black arrows indicate T-bet⁺Th1 cells. (c) Immunofluorescence staining for alpha-SMA⁺HSCs (red) and T-bet⁺Th1 cells (green) identified HSCs surrounded by Th1 cells. White arrows mark the positions of T-bet⁺Th1 cells