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. 2017 May 23;55(6):1658–1670. doi: 10.1128/JCM.01463-16

FIG 2.

FIG 2

Enrichment drastically increases the ratio of target pathogen nucleic acids to host nucleic acids in experimental samples. Quantitative RT-PCR results demonstrate an increase in the abundance of targeted nucleic acids (FIVC) and a decrease in nontargeted nucleic acids (PLVB and GAPDH) during the enrichment step in the protocol. *, PLVB was undetectable in postenrichment samples. Amp, amplification.