TABLE 3.
Results of two-tiered algorithms tested
| Sample (no.) | No. positive (%) |
||||
|---|---|---|---|---|---|
| Two-tiered testinga,b |
Two-EIA algorithms (MTTT) |
||||
| LYT-ViraStripe STTT | LYM/LYG-ViraStripe STTT | C6-ViraStripe STTT | LYT-C6 | LYM/LYG-C6c | |
| Lyme disease (124) | |||||
| Early Lyme disease with EM | |||||
| Acute (40) | 19 (48) | 17 (43) | 17 (43) | 20 (50) | 22 (55) |
| Convalescent (38) | 24 (63) | 23 (61) | 24 (63) | 30 (79) | 29 (76) |
| Lyme neuroborreliosis or Lyme carditis | |||||
| Lyme neuroborreliosis (10) | 8 (80) | 9 (90) | 9 (90) | 9 (90) | 10 (100) |
| Lyme carditis (7) | 7 (100) | 7 (100) | 6 (86) | 6 (86) | 6 (86) |
| Late Lyme disease, Lyme arthritis (29) | 28 (97) | 28 (97) | 28 (97) | 29 (100) | 29 (100) |
| All Lyme disease | 86 (69) | 84 (68) | 84 (68) | 94 (76) | 96 (77) |
| Negative controls (347) | |||||
| Other diseases (144) | |||||
| Fibromyalgia (31) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) |
| Severe periodontitis (20) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) |
| Rheumatoid arthritis (21) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) |
| Syphilis (20) | 0 (0) | 0 (0) | 0 (0) | 2 (10) | 1 (5) |
| Multiple sclerosis (22) | 0 (0) | 0 (0) | 0 (0) | 1 (5) | 0 (0) |
| Infectious mononucleosis (30) | 8 (27) | 5 (17) | 2 (7) | 2 (7) | 1 (3) |
| All other diseases | 8 (6) | 5 (3) | 2 (1) | 5 (3) | 2 (1) |
| Healthy controls (203) | |||||
| Endemicd (101) | 3 (3) | 5 (5) | 1 (1) | 1 (1) | 1 (1) |
| Nonendemicd (102) | 1 (1) | 1 (1) | 0 (0) | 0 (0) | 1 (1) |
| All healthy controls | 4 (2) | 6 (3) | 1 (0) | 1 (0) | 2 (1) |
| All negative controls | 12 (3) | 11 (3) | 3 (1) | 6 (2) | 4 (1) |
a
ViraStripe immunoblots were read using a densitometer.
b
A ≤30-day duration-of-illness cutoff was applied for IgM Western immunoblotting.
c
The same results were obtained whether or not the ≤30-day duration-of-illness cutoff was applied to the LYM EIA.
d
Endemic and nonendemic are as defined in the footnotes to Table 1.