TABLE 3.
Primers used for the multiplex PCR to identify to the species level and differentiate S. hyicus, S. agnetis, and S. aureus
| Genea | Primer | Sequence (5′ to 3′) | Amplicon size (bp) | Positive species |
|---|---|---|---|---|
| aroD | aroD-HyF | TAT GGT GTC GAC CAA TCG AAG GCT | 425 | S. hyicus |
| aroD | aroD-HyR | ACC CTA TAG CCC GCT TAC TT | ||
| aroD | aroD-AgF | CGC ATG AGA GAC CAA TAC GCT | 293 | S. agnetis |
| aroD | aroD-AgR | TAG GAC GTA TAG AGG TGG | ||
| nucb | nuc-S | CTG GCA TAT GTA TGG CAA TTG TT | 664 | S. aureus |
| nucb | nuc-AS | TAT TGA CCT GAA TCA GCG TTG TCT |
a
aroD, 3-dehydroquinate dehydratase gene; nuc, thermonuclease gene.
b
See reference 35.