Overall performance of Zika NS1-based ELISA. A total of 105 samples from ZIKV-infected travelers returning from areas where ZIKV is endemic were tested with IgM and IgG Zika NS1-based ELISA. Number of samples for each day post-symptom onset (A) and percentage of samples obtained 1 to 7, 8 to 26, 27 to 61, and >61 days post-symptom onset (B) are presented. Negative (Neg), positive (Pos), and equivocal IgM and IgG antibodies are indicated.