TABLE 1.
Capsular serotyping of C. canimorsus dog isolates by ELISA
| Strain/isolate |
Capsular serovara |
||||||||
|---|---|---|---|---|---|---|---|---|---|
| A | B | C | D | E | F | G | H | I | |
| Cc5 | 100 ± 0 | 20 ± 8 | 27 ± 11 | 24 ± 6 | 17 ± 2 | 13 ± 4 | 14 ± 5 | 10 ± 4 | 13 ± 5 |
| Cc5 Y1C12 | 14 ± 6 | ND | ND | ND | ND | ND | ND | ND | ND |
| Cc6 | 32 ± 7 | 100 ± 0 | 24 ± 12 | 20 ± 4 | 13 ± 2 | 14 ± 6 | 12 ± 3 | 11 ± 4 | 14 ± 7 |
| Cc6 ΔwbuB | ND | 14 ± 7 | ND | ND | ND | ND | ND | ND | ND |
| Cc9 | 15 ± 3 | 17 ± 7 | 100 ± 0 | 22 ± 4 | 17 ± 5 | 14 ± 5 | 13 ± 4 | 10 ± 3 | 14 ± 4 |
| Cc9 ΔwbuB | ND | ND | 20 ± 7 | ND | ND | ND | ND | ND | ND |
| Cc12 | 19 ± 7 | 15 ± 5 | 23 ± 8 | 100 ± 0 | 18 ± 0 | 14 ± 5 | 15 ± 5 | 10 ± 3 | 13 ± 5 |
| Cc12 ΔwbtA | ND | ND | ND | 20 ± 5 | ND | ND | ND | ND | ND |
| Cc4 | 16 ± 3 | 14 ± 3 | 30 ± 10 | 26 ± 5 | 100 ± 0 | 13 ± 5 | 13 ± 4 | 10 ± 2 | 13 ± 5 |
| CcD3 | 18 ± 7 | 11 ± 4 | 18 ± 5 | 19 ± 3 | 14 ± 6 | 10 ± 3 | 12 ± 1 | 15 ± 4 | 12 ± 6 |
| CcD5 | 17 ± 4 | 13 ± 8 | 16 ± 4 | 22 ± 4 | 13 ± 6 | 11 ± 5 | 13 ± 2 | 14 ± 6 | 14 ± 6 |
| CcD6 | 18 ± 10 | 11 ± 5 | 20 ± 7 | 18 ± 3 | 15 ± 7 | 11 ± 3 | 14 ± 2 | 17 ± 4 | 16 ± 10 |
| CcD10 | 17 ± 8 | 12 ± 5 | 18 ± 3 | 17 ± 2 | 17 ± 5 | 10 ± 1 | 14 ± 2 | 15 ± 6 | 14 ± 6 |
| CcD13 | 16 ± 7 | 11 ± 5 | 21 ± 4 | 17 ± 2 | 12 ± 5 | 99 ± 1 | 12 ± 1 | 13 ± 4 | 12 ± 5 |
| CcD16 | 18 ± 7 | 10 ± 4 | 17 ± 4 | 86 ± 14 | 11 ± 5 | 10 ± 3 | 13 ± 1 | 15 ± 5 | 12 ± 5 |
| CcD18 | 19 ± 9 | 12 ± 6 | 17 ± 5 | 15 ± 2 | 28 ± 12 | 10 ± 2 | 15 ± 1 | 17 ± 6 | 13 ± 6 |
| CcD20 | 17 ± 6 | 11 ± 6 | 19 ± 3 | 17 ± 3 | 57 ± 24 | 11 ± 3 | 12 ± 1 | 14 ± 4 | 12 ± 5 |
| CcD25 | 15 ± 6 | 11 ± 5 | 18 ± 4 | 17 ± 2 | 12 ± 5 | 9 ± 2 | 11 ± 1 | 14 ± 4 | 12 ± 3 |
| CcD33 | 20 ± 9 | 11 ± 6 | 17 ± 6 | 22 ± 2 | 13 ± 5 | 10 ± 3 | 12 ± 2 | 13 ± 4 | 106 ± 30 |
| CcD34 | 16 ± 9 | 10 ± 4 | 16 ± 3 | 14 ± 2 | 13 ± 6 | 10 ± 2 | 12 ± 1 | 13 ± 4 | 13 ± 6 |
| CcD35 | 14 ± 7 | 12 ± 4 | 15 ± 5 | 12 ± 1 | 12 ± 4 | 11 ± 3 | 12 ± 2 | 14 ± 3 | 12 ± 5 |
| CcD37 | 15 ± 4 | 9 ± 3 | 19 ± 2 | 16 ± 0 | 12 ± 4 | 100 ± 0 | 11 ± 1 | 12 ± 4 | 11 ± 5 |
| CcD39 | 14 ± 4 | 10 ± 5 | 18 ± 3 | 22 ± 2 | 14 ± 6 | 9 ± 2 | 11 ± 1 | 12 ± 3 | 12 ± 5 |
| CcD40 | 16 ± 8 | 11 ± 5 | 19 ± 4 | 19 ± 4 | 12 ± 5 | 10 ± 2 | 13 ± 2 | 16 ± 5 | 13 ± 6 |
| CcD43 | 20 ± 10 | 24 ± 14 | 86 ± 5 | 17 ± 1 | 13 ± 5 | 10 ± 3 | 12 ± 1 | 16 ± 1 | 14 ± 2 |
| CcD44 | 15 ± 8 | 9 ± 4 | 25 ± 7 | 16 ± 0 | 12 ± 5 | 8 ± 1 | 11 ± 1 | 12 ± 4 | 11 ± 5 |
| CcD47 | 16 ± 6 | 11 ± 6 | 17 ± 3 | 18 ± 0 | 12 ± 5 | 8 ± 2 | 12 ± 1 | 14 ± 3 | 11 ± 4 |
| CcD51 | 15 ± 7 | 14 ± 5 | 18 ± 4 | 20 ± 4 | 11 ± 5 | 9 ± 2 | 12 ± 0 | 14 ± 4 | 12 ± 5 |
| CcD52 | 16 ± 8 | 11 ± 7 | 16 ± 5 | 20 ± 6 | 11 ± 5 | 83 ± 4 | 13 ± 2 | 14 ± 4 | 12 ± 5 |
| CcD53 | 19 ± 8 | 12 ± 6 | 17 ± 2 | 18 ± 2 | 12 ± 5 | 9 ± 2 | 14 ± 2 | 41 ± 7 | 11 ± 4 |
| CcD57 | 17 ± 6 | 28 ± 18 | 21 ± 4 | 23 ± 12 | 32 ± 10 | 9 ± 2 | 13 ± 1 | 13 ± 4 | 12 ± 5 |
| CcD58 | 18 ± 7 | 11 ± 5 | 17 ± 3 | 22 ± 3 | 34 ± 11 | 11 ± 2 | 14 ± 1 | 15 ± 5 | 13 ± 5 |
| CcD63 | 15 ± 9 | 11 ± 5 | 17 ± 1 | 17 ± 0 | 29 ± 11 | 11 ± 5 | 100 ± 0 | 14 ± 5 | 12 ± 5 |
| CcD68 | 43 ± 7 | 110 ± 11 | 16 ± 5 | 18 ± 0 | 13 ± 5 | 9 ± 2 | 13 ± 1 | 14 ± 5 | 11 ± 5 |
| CcD69 | 14 ± 7 | 11 ± 6 | 16 ± 3 | 13 ± 2 | 12 ± 5 | 8 ± 3 | 12 ± 1 | 12 ± 4 | 11 ± 5 |
| CcD71 | 15 ± 6 | 11 ± 5 | 17 ± 6 | 16 ± 2 | 13 ± 6 | 10 ± 2 | 13 ± 1 | 14 ± 5 | 13 ± 5 |
| CcD73 | 19 ± 7 | 12 ± 7 | 17 ± 0 | 23 ± 3 | 15 ± 6 | 15 ± 7 | 13 ± 5 | 18 ± 7 | 13 ± 5 |
| CcD76 | 13 ± 6 | 16 ± 8 | 14 ± 1 | 15 ± 3 | 15 ± 7 | 15 ± 7 | 13 ± 4 | 12 ± 4 | 13 ± 4 |
| CcD77 | 14 ± 9 | 13 ± 4 | 17 ± 7 | 14 ± 2 | 15 ± 7 | 14 ± 6 | 12 ± 5 | 10 ± 2 | 13 ± 4 |
| CcD80 | 16 ± 11 | 13 ± 4 | 19 ± 5 | 17 ± 3 | 30 ± 9 | 13 ± 5 | 13 ± 5 | 10 ± 4 | 13 ± 5 |
| CcD81 | 16 ± 5 | 12 ± 4 | 23 ± 7 | 19 ± 4 | 22 ± 5 | 12 ± 5 | 12 ± 5 | 9 ± 3 | 11 ± 4 |
| CcD84 | 17 ± 8 | 14 ± 7 | 13 ± 1 | 18 ± 2 | 29 ± 16 | 12 ± 5 | 12 ± 3 | 14 ± 4 | 13 ± 4 |
| CcD89 | 17 ± 6 | 15 ± 1 | 20 ± 6 | 95 ± 9 | 14 ± 9 | 14 ± 5 | 13 ± 4 | 13 ± 5 | 13 ± 5 |
| CcD96 | 20 ± 8 | 14 ± 1 | 19 ± 7 | 15 ± 1 | 118 ± 37 | 12 ± 4 | 11 ± 4 | 9 ± 3 | 13 ± 5 |
| CcD101 | 18 ± 5 | 12 ± 3 | 13 ± 4 | 16 ± 1 | 13 ± 7 | 12 ± 5 | 10 ± 3 | 100 ± 0 | 12 ± 4 |
| CcD104 | 16 ± 9 | 14 ± 1 | 18 ± 7 | 21 ± 4 | 31 ± 11 | 11 ± 4 | 10 ± 3 | 9 ± 3 | 11 ± 4 |
| CcD105 | 107 ± 28 | 14 ± 2 | 16 ± 8 | 17 ± 5 | 14 ± 8 | 13 ± 5 | 11 ± 4 | 9 ± 3 | 12 ± 4 |
| CcD106 | 13 ± 8 | 16 ± 1 | 21 ± 11 | 17 ± 2 | 59 ± 24 | 13 ± 5 | 11 ± 4 | 9 ± 3 | 13 ± 5 |
| CcD113 | 15 ± 11 | 16 ± 2 | 19 ± 9 | 14 ± 0 | 14 ± 6 | 108 ± 6 | 11 ± 4 | 9 ± 4 | 12 ± 4 |
| CcD115 | 15 ± 9 | 16 ± 2 | 19 ± 9 | 21 ± 4 | 16 ± 8 | 12 ± 4 | 10 ± 4 | 9 ± 3 | 12 ± 5 |
| CcD116 | 14 ± 8 | 14 ± 2 | 19 ± 5 | 18 ± 2 | 15 ± 7 | 12 ± 5 | 12 ± 5 | 9 ± 3 | 13 ± 5 |
| CcD117 | 19 ± 10 | 16 ± 1 | 19 ± 8 | 99 ± 12 | 14 ± 7 | 13 ± 6 | 13 ± 5 | 9 ± 3 | 13 ± 5 |
| CcD118 | 15 ± 7 | 14 ± 1 | 20 ± 9 | 19 ± 4 | 15 ± 7 | 111 ± 5 | 12 ± 4 | 10 ± 3 | 12 ± 5 |
| CcD119 | 15 ± 8 | 16 ± 2 | 16 ± 9 | 28 ± 22 | 15 ± 9 | 12 ± 4 | 11 ± 4 | 12 ± 5 | 12 ± 4 |
| CcD120 | 15 ± 8 | 17 ± 3 | 17 ± 5 | 23 ± 7 | 16 ± 8 | 13 ± 5 | 12 ± 4 | 10 ± 3 | 12 ± 4 |
| CcD122 | 12 ± 8 | 14 ± 1 | 17 ± 6 | 13 ± 1 | 14 ± 8 | 13 ± 5 | 12 ± 4 | 9 ± 2 | 12 ± 4 |
| CcD124 | 15 ± 8 | 16 ± 1 | 19 ± 8 | 19 ± 4 | 15 ± 7 | 109 ± 9 | 11 ± 4 | 9 ± 3 | 12 ± 4 |
| CcD126 | 13 ± 7 | 14 ± 1 | 16 ± 6 | 21 ± 3 | 15 ± 7 | 13 ± 6 | 11 ± 4 | 11 ± 4 | 12 ± 4 |
| CcD129 | 13 ± 9 | 15 ± 2 | 21 ± 6 | 20 ± 2 | 16 ± 8 | 12 ± 5 | 14 ± 6 | 10 ± 2 | 100 ± 0 |
| CcD130 | 13 ± 7 | 29 ± 11 | 108 ± 26 | 19 ± 2 | 11 ± 6 | 15 ± 6 | 12 ± 4 | 10 ± 4 | 13 ± 5 |
| CcD131 | 12 ± 7 | 14 ± 2 | 17 ± 6 | 19 ± 3 | 14 ± 6 | 13 ± 6 | 11 ± 4 | 10 ± 3 | 12 ± 4 |
a
Capsular serotyping was determined by ELISA on entire heat-killed bacteria. The following sera were used: Y1C12-adsorbed anti-Cc5 (A), Cc6 ΔwbuB adsorbed anti-Cc6 (B), Cc9 ΔwbuB adsorbed anti-Cc9 (C), Cc12 ΔwbtA adsorbed anti-Cc12 (D), anti-Cc4 adsorbed with all human isolates except Cc4 (E), anti CcD37 adsorbed with all human isolates (F), anti CcD63 adsorbed with all human isolates (G), anti CcD101 adsorbed with all human isolates (H), and anti CcD129 adsorbed with all human isolates (I). The readout of the ELISA was absorbance, but the results are expressed here as percentage of reactivity calculated with respect to the absorbance value obtained for the capsular type strain. Values are the mean ± standard deviation (SD) of the results from at least 3 independent experiments. Isolates with reactivities ≥30% appear in bold face. In addition, isolates with reactivities higher than 80% are highlighted in gray. ND, not determined.