FIG 6.

Comparable liver pathology in R. typhi^GFPuv- and wild-type R. typhi-infected CB17 SCID mice. (A) Representative images of the livers of the animals shown in Fig. 4 and harvested from moribund mice after euthanasia demonstrate liver necrosis in mice infected with wild-type R. typhi in the absence of antibiotics and in animals that were infected with R. typhi^GFPuv, whether treated with rifampin (rif) or not (−). Livers of control mice that received PBS or were infected with wild-type R. typhi and were treated with rifampin appeared healthy. (B) Representative H&E staining of the liver of a rifampin-treated mouse infected with wild-type R. typhi (left), a mouse infected with wild-type R. typhi without antibiotic treatment (center), and a rifampin-treated mouse infected with R. typhi^GFPuv (right). Livers from rifampin-treated animals infected with wild-type R. typhi appeared healthy, while necrotic lesions and cellular infiltrates were detectable in the livers from untreated mice infected with wild-type R. typhi, as well as in mice infected with R. typhi^GFPuv. (C) The percentages of necrotic areas (left) and the mHAI were quantified. The graphs show means and SEM. Statistical analysis was performed using one-way ANOVA (Kruskall-Wallis test followed by Dunn's posttest). The asterisks indicate statistically significant differences (*, P < 0.05).