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Growth analysis of mutants in the presence of bile salts. The ΔacrB (A) and ΔgalU (B) mutants were analyzed for growth in TSB ± 0.4% and 2% (wt/vol) bile salts. The graphs depict the growth curve analysis of three independent experiments, with the average OD600 ± SE plotted. The mutants were unable to grow in either 0.4% or 2% bile salts (P < 0.001), and complementation restored growth in both 0.4% and 2% bile salts (P < 0.001).
