TABLE 2.
Bacterial strains and plasmids used in this study
| Name | Description | Source or reference |
|---|---|---|
| Strains | ||
| Shigella | ||
| 2457T | S. flexneri serotype 2a | 76 |
| M90T | S. flexneri serotype 5 | 77 |
| 1617 | S. dysenteriae | 78 |
| AF6 | S. sonnei (recent clinical isolate) | AFRIMSa |
| BS766 | 2457T transformed with pKM208 | 70 |
| ΔacrB | 2457T/acrB::cat, chloramphenicol resistance | This study |
| ΔacrB (pAcrB) | ΔacrB transformed with pAcrB | This study |
| BS109 | 2457T/galU::Tn10, tetracycline resistance | 26 |
| BS109 (pGalU) | BS109 transformed with pGalU | This study |
| E. coli | ||
| DH5α | Laboratory E. coli | 79 |
| F18 | Mouse commensal E. coli | 80 |
| HS | Human commensal E. coli | 81 |
| MC4100 | E. coli K-12 derivative | 82 |
| 86-24 | Enterohemorrhagic E. coli O157:H7 (Stx2) | 83 |
| 933 | Enterohemorrhagic E. coli O157:H7 (Stx1, Stx2) | 83 |
| STEC 98NK2 | Shiga toxin-producing E. coli O113:H21 strain | 84 |
| EPEC E2348/69 | Enteropathogenic E. coli archetype O127:H6 strain | 81 |
| EAEC O42 | Enteroaggregative E. coli archetype O42 | 85 |
| Δaaf strain | O42/aafA::TnphoA | 86 |
| Salmonella | ||
| Typhi | S. enterica serovar Typhi strain Ty2 | 87 |
| Typhimurium | S. enterica serovar Typhimurium strain SL1344 | 88 |
| Plasmids | ||
| pKD3 | oriR6K, bla, cat | 89 |
| pKM208 | Temperature-sensitive red-, gam-, lacI-expressing plasmid driven by PTac promoter, bla | 89 |
| pUC19 | Cloning vector, bla, high copy no. | 90 |
| pGEMT | PCR cloning vector, bla, high copy no. | Promega |
| pAcrB | pUC19::acrB, wild-type gene cloned into pUC19 | This study |
| pGalU | pGEMT::galU, wild-type gene cloned into pGEMT | This study |
a
Department of Enteric Diseases, Armed Forces Research Institute of Medical Sciences (AFRIMS), Bangkok, Thailand.