FIG 3.

The agr operon positively regulates in vitro NetB production in CP1 via the Agr-like QS system. (A) Western blot analyses of NetB production in wild-type CP1, agrB, luxS, and virR mutants, and complemented strains. Total proteins (100 μg) from supernatants derived from TPG cultures (OD₆₀₀ = 0.8) of wild-type CP1, isogenic mutants, and complemented strains were separated by 10% SDS-PAGE, and Western blotting was performed with a mouse anti-NetB primary monoclonal antibody and goat anti-mouse AP-conjugated IgG secondary antibody. (B) qPCR analyses of netB expression in wild-type CP1, agrB, luxS, and virR mutant, and complemented strains in TPG culture (OD₆₀₀ = 0.8). The data are expressed as the means ± standard deviations (SD) from three separate experiments. *, P < 0.05 versus the CP1 group.