FIG. 3.

Detection of P. piscicida dinospores emerged from sediment with (w/) and without (wo/) fish by species-specific PCR. The emergence of P. piscicida from Hyrock Farm pond 10 sediment with fish (top panel) or without fish (bottom panel) was examined in a flask bioassay format. Water samples (1 and 15 ml) were collected for detection of P. piscicida and counting of Pfiesteria-like dinoflagellates, respectively, following the time course (days 0.5, 1, 2, 3, 4, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, and 29).