FIG 1.

Depletion of alveolar macrophages increases susceptibility to LVS. (A) BALB/c mice were treated i.n. with clodronate liposomes or PBS liposomes on days 2 and 1 prior to infection, and depletion of alveolar macrophages in BALF was confirmed by flow cytometry. (B and C) At 2 days after treatment, mice (8 mice/group) were infected i.n. with 500 CFU (B) or 100 CFU (C) of F. tularensis LVS. Survival was monitored for 21 days. P values were determined by the log-rank test. **, P < 0.01; ***, P < 0.001.