FIG 3.

Neutrophil recruitment requires stimulation of CD68⁺ cells by IFN-γ. (A to C) MIIG and WT C57BL/6 mice were infected i.n. with 1 LD₅₀ of F. tularensis LVS (5 mice/group, except 3 mice/group for uninfected MIIG mice). At the indicated time points, lung Ly6G⁺ CD11b⁺ cells were enumerated by flow cytometry. (A) Representative flow cytometry plots of MIIG and WT mouse lungs after gating on live cells; (B) total lung neutrophils; (C) total viable cells. *, P < 0.05 for genotype by two-way ANOVA.