FIG 5.

The protective effect of IL-12 treatment requires NADPH oxidase but not neutrophils. (A) BALB/c mice (8 mice/group) were treated i.n. with IL-12 or PBS and given i.p. injections of the 1A8 MAb or an isotype-matched IgG. Mice were then infected i.n. with 1 LD₁₀₀ of F. tularensis LVS. Survival was monitored for 21 days. (B and C) Survival between WT C57BL/6 mice (8 mice/group) and p47^phox−/− mice (5 mice/group) (B) or gp91^phox−/− mice (6 mice/group) (C) was compared after i.n. treatment with IL-12 or PBS 1 day prior to i.n. infection with 1 LD₁₀₀ CFU of F. tularensis LVS. Survival was monitored for 21 days. P values for the significance of the differences between the indicated groups were determined by the log-rank test. **, P < 0.01; ***, P < 0.001; NS, not significant. KO, knockout.