TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Antibiotic resistancea | Source or reference(s) |
|---|---|---|---|
| Strains | |||
| H. pylori J166 | WT clinical isolate | 23, 27 | |
| H. pylori J166 babA::cat | J166 with cat inserted in babA | Cm | This study |
| H. pylori J166 babB::cat | J166 with cat inserted in babB | Cm | This study |
| H. pylori J166 babA-CL2 | J166 with cysteines 189 and 197 replaced with alanines in babA | Str | 13 |
| H. pylori J166 babBB | J166 with babA replaced with second copy of babB | Str | This study |
| E. coli Top10 | Cloning strain | Invitrogen | |
| Plasmids | |||
| pBluescript SK− | Cloning vector | Ap | Agilent |
| pJ272 | J166 babA, including 1,459 bp upstream and 1,173 bp downstream cloned into pBluescript SK−, bp 167–1335 of babA replaced with chloramphenicol resistance cassette | Cm | This study |
| pJ270 | J166 babB, including 1,510 bp upstream and 1,919 bp downstream cloned into pBluescript SK−, bp 167–1227 of babB replaced with chloramphenicol resistance cassette | Cm | This study |
| pJ231 | Bp 1638–312 upstream of J166 babA joined to chloramphenicol resistance cassette and bp 1343–2224 of babA cloned into pBluescript SK− | Cm | This study |
| pJ232 | Bp 1638–312 upstream of J166 babA joined to kanamycin resistance cassette and bp 1–2116 of babB, including 348 bp upstream, cloned into pBluescript SK− | Km | This study |
a
Cm, chloramphenicol; Str, streptomycin; Ap, ampicillin; Km, kanamycin.