TABLE 2.

Primers used for PCR, cloning, and sequencing

Primer purpose and name	Sequence (5′ to 3′)a
Construction of babA::cat
HP0898:930U24_NotI	AAC GCGGCCGC TTT AAG CCA CAA AAC CTC TAA AGA
HP0896:148L25_HincII	AAC GTCGAC GAT TGT TCA GCT TTT CAT AAT TGT C
CAT_F_SacI	AAC GAGCTC GCG GAC AAC GAG TAA AAG AG
CAT_R_HincII	AAC GTCGAC GCA GGA CGC ACT ACT CTC G
HP0896:1234U23_SacI	AAC GAGCTC TAT GTG GAA CAA ACC ATA ACG AA
JHP0830:224L22_XhoI	AAT CTCGAG TCA GCA TTC ACT TCC TTT TTG A
Construction of babB::cat
JHP1167:383U18_NotI	AAC GCGGCCGC CTC ACC ACG CAG AGG AAG
HP0896:148L25_PstI	AAC CTGCAG GAT TGT TCA GCT TTT CAT AAT TGT C
CAT_F_SacI	AAC GAGCTC GCG GAC AAC GAG TAA AAG AG
CAT_R_PstI	AAC CTGCAG GCA GGA CGC ACT ACT CTC G
HP0896:1234U23_SacI	AAC GAGCTC TAT GTG GAA CAA ACC ATA ACG AA
HP1241:772L20_XhoI	AAT CTCGAG TGG AAC TCG CTC GCA TAA TC
Construction of pJ231
HP0898:751U24_NotI	AAC GCGGCCGC ATC CAA TAC AAA AGA GCG GTG AGC
BabAR19_SacI	AAC GAGCTC TTA TCG CTT GCT TGA TGC AAG CTC
CAT_F_HincII	AAC GTCGAC GCG GAC AAC GAG TAA AAG AG
CAT_R_SacI	AAC GAGCTC GCA GGA CGC ACT ACT CTC G
BabAF20_HincII	AAC GTCGAC CAA CAA ACC ATA ACG AAT TTA ACC AAC AG
HP0896:2097L26_XhoI	AAC CTCGAG AAG CGA ACA CGT AAT TCA AAT ACA CG
Construction of pJ232
HP0898:751U24_NotI	AAC GCGGCCGC ATC CAA TAC AAA AGA GCG GTG AGC
BabAR19_HincII	AAC GTCGAC TTA TCG CTT GCT TGA TGC AAG CTC
Kan_F_SacI	AAC GAGCTC GGT ACC CGG GTG AC
Kan_R_HincII	AAC GTCGAC TCT AGA GGA TCC CC
BabBF9_SacI	AAC GAGCTC GAT TTT AGC GGT GAT TTC TTG AGC G
HP0896:2097L26_XhoI	AAC CTCGAG AAG CGA ACA CGT AAT TCA AAT ACA CG
Amplification and sequencing of babA locus
BabAF14	GCA TCA AGC AAG CGA TAA CTT TAC TAA
P160R	ATA CCC TGG CTC GTT GTT GAA
P178R	CAT GTC CTG GCT CAT AAT ACG AA
CAT_reverse	CCC TGC ACA TAT AGT ATG ACG GTA A
BabAF19	ATG CTA TAA TAC TCC AAA TAC ATT CCA A
Amplification and sequencing of babB locus
BabBF3	CTT GAG CGT TAT TAG AAA TCT AGT GG
P178R	CAT GTC CTG GCT CAT AAT ACG AA
CAT_reverse	CCC TGC ACA TAT AGT ATG ACG GTA A

^aRestriction sites are underlined.