FIG 7.
The cell-permeabilizing and AC enzyme activities of CyaA synergize in provoking reduction of MHC-II molecule expression on myeloid cells in infected lungs. Expression of the MHC-II molecule on selected cell subsets in lungs of BALB/c mice infected with B. pertussis 6 days after infection with 1.5 × 105 CFU of the various strains or with medium (Control). (A) Histograms for one representative mouse lung suspension per experimental group. (B) Total counts of MHC-II-expressing cells per million lung cells (n = 5). (C) Mean level (MFI) of MHC-II molecules detected on the surface of selected cell subsets in the infected lungs (n = 5). Groups were compared using ANOVA followed by Tukey test for pairwise comparison of subgroups. *, **, and *** represent P values of <0.05, <0.01, and <0.001, respectively. The experiment was repeated twice with similar results. Mϕ, macrophages; MONO, monocytes; cDC, conventional dendritic cells; pDC, plasmacytoid dendritic cells.