FIG 2.
PmtA is critical for GAS growth under iron toxicity. (A) Transcript levels of pmtA in the indicated strains as measured by qRT-PCR. (B) Growth kinetics of the indicated strains in THY-C medium supplemented with increasing concentrations of FeSO4. Three biological replicates were performed, and the graph represents means ± standard deviations. Compl, complemented strain. (C) GAS strains were grown to mid-exponential phase in THY-C broth, incubated with 4 mM FeSO4 for 15 min, and grown for an additional 3 h in THY broth. The mean CFU recovered after a 3-h recovery period are shown, with P values as determined by a t test. Triplicate cultures were grown under the indicated conditions, and each biological replicate was assessed in duplicate. *, P < 0.0001.