FIG 4.

Upregulation of pmtA is dependent on the metallated state of PerR. (A) Transcript levels of pmtA in the indicated GAS strains grown in THY-C medium supplemented with 1 mM Fe(II) compared to those in untreated WT GAS as measured by qRT-PCR. (B) Transcript level analysis of pmtA in WT GAS grown in medium supplemented with 0.25 mM FeSO₄ and challenged with increasing concentrations of Mn(II). (C) Transcript level analysis of pmtA in WT GAS and the ΔperR mutant supplemented with 0.25 mM FeSO₄ and challenged with increasing concentrations of Mn(II). Three biological replicates were performed and analyzed in triplicate. Data graphed are means ± standard deviations. Average values for the growth of the WT in unsupplemented medium were used as a reference, and fold changes in the transcript levels in the indicated strains relative to those in the reference sample are shown. *, P < 0.0001.