FIG 11.
A strain with decreased Suf function has decreased survival in neutrophils. The WT, sufD*, and lacB::Tn strains were opsonized with 20% human serum, washed, and then diluted to 2.5 × 107 CFU/ml and used to infect 250,000 PMNs per well in a 96-well plate. The neutrophils were lysed upon addition of 1% saponin, and CFU were determined at various time points by plating. The data presented represent the averages of biological triplicates, with error bars representing standard errors of the mean. Student t tests (two tailed) were performed on the data, and P values are shown for the sufD* strain relative to the WT.