FIG 4.

Iron-sulfur cluster-requiring proteins have decreased activity in S. aureus strains with decreased suf transcription. (A) AcnA activity was assessed in the WT, sufD*, and sufD* suf⁺ strains. (B) AcnA activity is decreased in the sufD* strain irrespective of culture aeration. The AcnA assays were conducted in cell lysates from the WT, sufD*, sodA::Tn, and ΔacnA strains cultured in TSB with altered HV ratios. (C) Glutamate dehydrogenase activities were assessed in the WT and sufD* strains. The data shown represent the averages of biological triplicates with standard deviations. *, P < 0.5 relative to the WT strain using a two-tailed Student t test.