FIG 8.

Decreased Suf function results in increased sensitivity to RNS and ROS. (A) Methyl viologen sensitivity was monitored in the WT, sufD*, sufD* suf⁺, and sodA::Tn strains. The cells were cultured in TSB before serial dilution and spot plating on solid TSA supplemented with 40 mM methyl viologen or vehicle control. (B) H₂O₂ sensitivity was assessed in the WT, sufD*, sufD* suf⁺, and katA::Tn strains. The cells were challenged with 500 mM H₂O₂ before the reaction was quenched, and the cells were serially diluted and spot plated on solid TSA medium. (C) Nitroprusside (NP) sensitivity was assessed in the WT, sufD*, and sufD* suf⁺ strains. Representative growth profiles in the presence and absence of 15 mM nitroprusside in TSB medium are shown. The data are from representative experiments.