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. 2005 Jan;71(1):72–79. doi: 10.1128/AEM.71.1.72-79.2005

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Copyright © 2005, American Society for Microbiology

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FIG. 1. — (a) Representative results for UG₅₂-Uc₅₃ (435-bp) amplicons and 292-222 (350-bp) amplicons for isolates A1, A2, A3, and A4. Lane M contained a molecular weight marker (φX174 HaeIII-generated restriction fragments; Gibco BRL). (b) Representative results of RFLP analysis of UC₅₃-UG₅₂-produced RT-PCR amplicons of the enterovirus isolates with the HpaII restriction endonuclease (for a description, see the text). All of the isolates except one (isolate A5 [data not shown]) belonged to the same genetic subcluster, and the restriction profile comprised four HpaII-generated fragments with lengths of 213, 149, 55, and 18 nucleotides. The lane on the left contained a molecular weight marker (φX174 HaeIII-generated restriction fragments; Gibco BRL).