FIG 4.
The absence of PtsP affects the expression levels of PmrA-regulated genes. The expression of effector translational lacZ fusions regulated by CpxR (A), PmrA (B), and CsrA (C) as well as sRNA transcriptional lacZ fusions regulated by LetA (D) (the genes examined are indicated below the bars) were examined in the wild-type strain (JR32) and in the ptsP deletion mutant (SY-ΔptsP) at the stationary phase. β-Galactosidase activity was measured as described in Materials and Methods. The levels of expression of the lacZ fusions were found to be significantly different (*, P < 10−5, Student's t test) in comparisons between results in the wild-type strain and those in the pmrA deletion mutant. Data (expressed in Miller units [M.U.]) are the averages ± standard deviations (error bars) of the results of at least three different experiments.