FIG 7.
The PTSNtr affects the levels of expression of PmrA-regulated genes and is independent of PmrB. The expression of effector translational lacZ fusions (the effectors examined are indicated below the bars) was examined in the wild-type strain (JR32), in the ptsP deletion mutant (SY-ΔptsP), in the pmrB deletion mutant (EA-pmrB), and in the ptsP-pmrB double deletion mutant (SY-ΔptsP-pmrB-Km). The levels of expression of the marked lacZ fusions were found to be significantly different (*, P < 0.001; **, P < 10−5, Student's t test). β-Galactosidase activity was measured as described in Materials and Methods. Data (expressed in Miller units [M.U.]) are the averages ± standard deviations (error bars) of the results of at least three different experiments.