FIG. 3.

(A) Map of the 4-kb SalI fragment containing mini-Tn5-lacZ₁-cat in mutant 5E4. The speckled box indicates a region where the sequence is unresolved. The arrows indicate the directions of ORFs; the corresponding gene designations are indicated below the arrows (at, acetyltransferase gene). The dashed arrow indicates the partial ORF for trpC. The open triangle indicates the position of the transposon insertion in 5E4. The striped boxes above the open triangle indicate deleted regions in the clp and clp-AT loci in DC211 and DCA2422, respectively. The open boxes indicate the positions of RT-PCR regions when the CAF1-CAR1 and CAF2-CAR2 primer sets were used. (B) Deduced amino acid sequence of the acetyltransferase, with the signature motifs (motifs A to D) of GNAT superfamily members highlighted. The residues in boldface type with solid circles above them are highly conserved, and the underlined residues are moderately conserved.