FIG. 4.

RT-PCR products from L. enzymogenes wild-type strain C3 (lanes 1 to 5) and mutant strain 5E4 (lanes 6 to 10) when constructed primers that span the clp and acetyltransferase ORFs were used. The CAF1-CAR1 primer set was used for lanes 1, 4, 6, and 9, and the CAF2-CAR2 primer set was used for lanes 2, 7, 5, and 10. Lanes 4, 5, 9, and 10 contained DNA-negative controls for the primer sets. Lanes 3 and 8 contained RT-PCR-positive controls in which primers for a gene (sctV) known to be expressed independent of the clp and acetyltransferase genes in L. enzymogenes were used.